Cells, Free Full-Text

Cancer stem cells play a crucial role in tumor initiation, metastasis, and resistance to treatment. Cellular heterogeneity and plasticity complicate the isolation of cancer stem cells. The impact of intra-tumor cellular heterogeneity using a label-free approach remains understudied in the context of treatment resistance. Here, we use the sedimentation field–flow fractionation technique to separate, without labeling, cell subpopulations of colorectal cancer cell lines and primary cultures according to their biophysical properties. One of the three sorted cell subpopulations exhibits characteristics of cancer stem cells, including high tumorigenicity in vivo and a higher frequency of tumor-initiating cells compared to the other subpopulations. Due to its chemoresistance, two- and three-dimensional in vitro chemosensitivity assays highlight the therapeutic relevance of this cancer stem cell subpopulation. Thus, our results reveal the major implication of intra-tumor cellular heterogeneity, including cancer stem cells in treatment resistance, thanks to our label-free cell sorting approach. This approach enables—by breaking down the tumor—the study the individualized response of each sorted tumor cell subpopulation and to identify chemoresistance, thus offering new perspectives for personalized therapy.

Mol Imaging Radionucl Ther on X: The Role of a Bone SPECT/CT Scan

THE LIVES OF A CELL : LEWIS THOMAS : Free Download, Borrow, and

The cell-free system: A new apparatus for affordable, sensitive

Cell-free Macromolecular Synthesis

Textile-embedded cell-free biosensors

Cell-Free Protein Synthesis: Methods and Protocols

Biology, Free Full-Text

Anomalous Scaling of Gene Expression in Confined Cell-Free

Advancing synthetic biology through cell-free protein synthesis

Cancer type classification using plasma cell-free RNAs derived

JCM, Free Full-Text

Nature Reviews Molecular Cell Biology on X: From May cover

Labile coat: reason for noninfectious cell-free varicella-zoster

Rapid cell-free forward engineering of novel genetic ring